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normal mouse serum  (Rockland Immunochemicals)


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    Structured Review

    Rockland Immunochemicals normal mouse serum
    Normal Mouse Serum, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+mouse+serum/NORMAL+MOUSE+SERUM/bio_rxiv__64898__2026__03__27__714269-189-18-21
    Average 94 stars, based on 12 article reviews
    normal mouse serum - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: A potential pathogenic association between periodontal disease and Crohn’s disease
    Article Snippet: .. Bacterial suspensions were incubated in a staining buffer containing 20% Normal Mouse Serum (Rockland) for 20 minutes. .. Antibody-coated bacteria were then stained with PE-labeled anti-human IgA (1:50, Miltenyi Biotec, clone IS11-8E10; mouse IgG1κ) or PE-labeled isotype control (IgG1κ; BioLegend) for 30 minutes.

    Staining:

    Article Title: A potential pathogenic association between periodontal disease and Crohn’s disease
    Article Snippet: .. Bacterial suspensions were incubated in a staining buffer containing 20% Normal Mouse Serum (Rockland) for 20 minutes. .. Antibody-coated bacteria were then stained with PE-labeled anti-human IgA (1:50, Miltenyi Biotec, clone IS11-8E10; mouse IgG1κ) or PE-labeled isotype control (IgG1κ; BioLegend) for 30 minutes.

    Article Title: A novel CCR5-specific pharmacodynamic assay in whole blood using phosphoflow cytometry highlights different ligand-dependent responses but similar properties of antagonists in CD8+ and CD4+ T lymphocytes.
    Article Snippet: Chemokine CC motif receptor (CCR) 5 is a major drug target for both inflammation and virology indications.. The primary function of CCR5 is to mediate the trafficking of CCR5-expressing lymphocytes to any of the CCR5 ligands, which are often increased during inflammatory responses.. In addition, CCR5 is a coreceptor for HIV, mediating R5 tropic HIV infection of CCR5-expressing CD4 T cells.

    Article Title: Fc receptor dependent and independent mechanisms of antibody-mediated enhancement of immune responses
    Article Snippet: .. For staining of slices, blocking was performed for 1 hour using Fc block (BD Biosciences 553142) and 5% normal mouse serum (Rockland B308) in 1x PBS containing .3% Triton X-100, .2% BSA and .1% sodium azide. ..

    Control:

    Article Title: HLA-DQA2 and HLA-DQB2 genes are specifically expressed in human Langerhans cells and encode a new HLA class II molecule.
    Article Snippet: The directly conjugated mouse mAbs used were: A488-conjugated antiCD207 (DCGM4; Dendritics, Lyon, France), FITC-conjugated anti-CD1a (BL6; Beckman-Coulter, Villepinte, France), A647-conjugated anti–HLA-DR (L243; Biolegend, San Diego, CA), allophycocyanin-conjugated anti-CD3 (Miltenyi Biotec, Paris, France), and Cy3-conjugated anti-CD63 (H5C6) (13). .. Control Igs were: mouse IgG1 (679.1 Mc7) and IgG2a (U7.27; Beckman Coulter), normal mouse serum (Rockland, Gilbertsville, PA), polyclonal rabbit IgGs (Southernbiotech, Birmingham, AL), and normal preimmune rabbit serum (Proteogenix, Oberhausbergen, France). .. To obtain anti–HLA-DQa2 and HLA-DQb2 mAbs, peptides from HLADQa2 (MAVGKHTLEFMM) and HLA-DQb2 (CRDFPKDFLVQFKG) were synthesized, coupled to keyhole limpet hemocyanin (Proteogenix, Oberhausbergen, France) and used in our laboratory to generate mouse anti-peptide mAbs.

    Concentration Assay:

    Article Title: Analysis of Lanthanide Complex Dendrimer Conjugates for Bimodal NIR and MRI Imaging
    Article Snippet: .. Lyophilized (Labconco Freeze Dry System, Model 775000, FreeZone 4.5 bench top and console freeze-dry systems) LnNX-EA conjugates were dissolved in Millipore water, the buffered solution (HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH = 7.4 and I = 0.1 M NaCl) or mouse serum (Rockland, Normal Mouse Serum, Lot #22406) at a concentration of 1.0 mg/1 mL. .. Solution for photoluminescent lifetime measurements in deuterated buffer, were prepared by lyophilizing solutions containing the complex to dryness and redissolving in 99.9 % deuterium oxide (Cambridge Isotope Laboratories, Inc) with a final concentration of 1 mg/mL.

    Article Title: Essential Role of Surface-Bound Complement Factor H in Controlling Immune Complex–Induced Arthritis
    Article Snippet: .. Alternative pathway hemolytic assay Lysis of sheep erythrocytes (E S ) was measured by mixing, on ice, gelatin veronal buffer (5 mM veronal, 145 mM NaCl, 0.02% NaN 3 [pH 7.3], 0.1% gelatin), normal mouse serum (40% final; Rockland Immunochemicals), and 0.1 M MgEGTA (5 mM final concentration) in the presence of buffer alone, 0–259 μg/ml murine rfH19-20 (0–18 μM), or 0–389 μg/ml human rfH3-5 (0–18 μM). ..

    Article Title: Analysis of Lanthanide Complex Dendrimer Conjugates for Bimodal NIR and MRI Imaging
    Article Snippet: .. Sample preparation Lyophilized (Labconco Freeze Dry System, Model 775000, FreeZone 4.5 bench top and console freeze-dry systems) LnNX-EA conjugates were dissolved in Millipore water, the buffered solution (HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH = 7.4 and I = 0.1 M NaCl) or mouse serum (Rockland, Normal Mouse Serum, Lot #22406) at a concentration of 1.0 mg/1 mL. .. Solution for photoluminescent lifetime measurements in deuterated buffer, were prepared by lyophilizing solutions containing the complex to dryness and redissolving in 99.9 % deuterium oxide (Cambridge Isotope Laboratories, Inc) with a final concentration of 1 mg/mL.

    Article Title: Essential Role of Surface-Bound Complement Factor H in Controlling Immune Complex–Induced Arthritis
    Article Snippet: .. Lysis of sheep erythrocytes (E S ) was measured by mixing, on ice, gelatin veronal buffer (5 mM veronal, 145 mM NaCl, 0.02% NaN 3 [pH 7.3], 0.1% gelatin), normal mouse serum (40% final; Rockland Immunochemicals), and 0.1 M MgEGTA (5 mM final concentration) in the presence of buffer alone, 0–259 μg/ml murine rfH19-20 (0–18 μM), or 0–389 μg/ml human rfH3-5 (0–18 μM). ..

    Blocking Assay:

    Article Title: A novel CCR5-specific pharmacodynamic assay in whole blood using phosphoflow cytometry highlights different ligand-dependent responses but similar properties of antagonists in CD8+ and CD4+ T lymphocytes.
    Article Snippet: Chemokine CC motif receptor (CCR) 5 is a major drug target for both inflammation and virology indications.. The primary function of CCR5 is to mediate the trafficking of CCR5-expressing lymphocytes to any of the CCR5 ligands, which are often increased during inflammatory responses.. In addition, CCR5 is a coreceptor for HIV, mediating R5 tropic HIV infection of CCR5-expressing CD4 T cells.

    Article Title: Fc receptor dependent and independent mechanisms of antibody-mediated enhancement of immune responses
    Article Snippet: .. For staining of slices, blocking was performed for 1 hour using Fc block (BD Biosciences 553142) and 5% normal mouse serum (Rockland B308) in 1x PBS containing .3% Triton X-100, .2% BSA and .1% sodium azide. ..

    Binding Assay:

    Article Title: A novel CCR5-specific pharmacodynamic assay in whole blood using phosphoflow cytometry highlights different ligand-dependent responses but similar properties of antagonists in CD8+ and CD4+ T lymphocytes.
    Article Snippet: Chemokine CC motif receptor (CCR) 5 is a major drug target for both inflammation and virology indications.. The primary function of CCR5 is to mediate the trafficking of CCR5-expressing lymphocytes to any of the CCR5 ligands, which are often increased during inflammatory responses.. In addition, CCR5 is a coreceptor for HIV, mediating R5 tropic HIV infection of CCR5-expressing CD4 T cells.

    Hemolytic Assay:

    Article Title: Essential Role of Surface-Bound Complement Factor H in Controlling Immune Complex–Induced Arthritis
    Article Snippet: .. Alternative pathway hemolytic assay Lysis of sheep erythrocytes (E S ) was measured by mixing, on ice, gelatin veronal buffer (5 mM veronal, 145 mM NaCl, 0.02% NaN 3 [pH 7.3], 0.1% gelatin), normal mouse serum (40% final; Rockland Immunochemicals), and 0.1 M MgEGTA (5 mM final concentration) in the presence of buffer alone, 0–259 μg/ml murine rfH19-20 (0–18 μM), or 0–389 μg/ml human rfH3-5 (0–18 μM). ..

    Lysis:

    Article Title: Essential Role of Surface-Bound Complement Factor H in Controlling Immune Complex–Induced Arthritis
    Article Snippet: .. Alternative pathway hemolytic assay Lysis of sheep erythrocytes (E S ) was measured by mixing, on ice, gelatin veronal buffer (5 mM veronal, 145 mM NaCl, 0.02% NaN 3 [pH 7.3], 0.1% gelatin), normal mouse serum (40% final; Rockland Immunochemicals), and 0.1 M MgEGTA (5 mM final concentration) in the presence of buffer alone, 0–259 μg/ml murine rfH19-20 (0–18 μM), or 0–389 μg/ml human rfH3-5 (0–18 μM). ..

    Article Title: Essential Role of Surface-Bound Complement Factor H in Controlling Immune Complex–Induced Arthritis
    Article Snippet: .. Lysis of sheep erythrocytes (E S ) was measured by mixing, on ice, gelatin veronal buffer (5 mM veronal, 145 mM NaCl, 0.02% NaN 3 [pH 7.3], 0.1% gelatin), normal mouse serum (40% final; Rockland Immunochemicals), and 0.1 M MgEGTA (5 mM final concentration) in the presence of buffer alone, 0–259 μg/ml murine rfH19-20 (0–18 μM), or 0–389 μg/ml human rfH3-5 (0–18 μM). ..

    Sample Prep:

    Article Title: Analysis of Lanthanide Complex Dendrimer Conjugates for Bimodal NIR and MRI Imaging
    Article Snippet: .. Sample preparation Lyophilized (Labconco Freeze Dry System, Model 775000, FreeZone 4.5 bench top and console freeze-dry systems) LnNX-EA conjugates were dissolved in Millipore water, the buffered solution (HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH = 7.4 and I = 0.1 M NaCl) or mouse serum (Rockland, Normal Mouse Serum, Lot #22406) at a concentration of 1.0 mg/1 mL. .. Solution for photoluminescent lifetime measurements in deuterated buffer, were prepared by lyophilizing solutions containing the complex to dryness and redissolving in 99.9 % deuterium oxide (Cambridge Isotope Laboratories, Inc) with a final concentration of 1 mg/mL.



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